The Zebrafish: Biology presents laboratory protocols and reviews that focus on Danio rerio, the tropical, freshwater fish that has emerged as an extremely powerful molecular-genetic model system for analysis of vertebrate development. This volume describes the embryology of the zebrafish, presents methods for cell culture and for the study of gene expression and function in development, highlights cellular techniques for analysis of morphogenesis, and concludes with protocols for the study of and descriptions of mutations that perturb organogenesis. Accessible to novices and "old hands" alike, this volume summarizes all aspects of zebrafish cell and developmental biology.
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