This successful laboratory handbook focuses on procedures for hybridoma generation and gives detailed test protocols for the application of monoclonal antibodies. Immunization, cell preparation, hybridization, cell culture and cloning of hybridomas, prevention of back-mutation, as well as purification, labeling, and characterization of the antibodies obtained, are only a few keywords regarding the contents. Practical hints and detailed product information together with detailed protocols for trouble-shooting make this book an exceptionally useful "cookbook" as well as a compendium of immunological methods over and above monoclonal antibody technology. The authors have emphasized protocols which tend to minimize the number of animals used and favor methods which avoid radioactivity.
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