Vectors: Expression Systems Essential Techniques

The use of specialized cloning vectors that facilitate the cloning, characterization and subsequent manipulation of DNA fragments has revolutionized molecular biology. Initially focusing on E. coli as a model system, Vectors: Expression Systems describes expression techniques for optimized targeting and purification of proteins. Specific eukaryotic or eukaryote-based expression systems are then detailed. Together with its companion volume, Vectors: Cloning Applications, which describes the basic cloning procedures that must be mastered before DNA characterization or manipulation can be attempted, it is essential reading for all molecular biologists. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.