Nuclease Methods and Protocols

Processivity of DNA repair enzymes. (R. Stephen Lloyd). Analysis by HLPC of distributive activities and the synthetic (back) reaction of pancreatic-type ribonucleases. (M. Victória Nogués and Claudi M. Cuchillo). Ire 1 p: a kinase and site-specific endoriconuclease. (Tania N. Gonzales and Peter Walter). Microtiter-plate assay and related assays for nonspecific endonucleases. (Gregor Meiss, Oleg Gimadutdinow, Peter Friedhoff, and Alfred M. Pingoud). Quantitating mRNAs with relative and competitive RT-PCR. (Ellen A. Prediger). Expressing self-incompatibility RNases (S-RNases) in transgenic plants. (Brian Beecher and Bruce A. McClure). Molecular cloning, tissue distribution, and chromosomal localization of the human homolog of the R2/Th/Stylar ribonuclease gene family. (Francesco Acquati, Cinzia Nucci, Marco G. Bianchi, Tatiana Gorletta, Roberto Taramelli). Ribonuclease inhibitors. (Brittan L. Paloske). Producing solube recombinant RNases and assays to measure their interaction with interferon-y in vitro. (Catherine H. Schein). Assays for the evaluation of HIV-1 integrase inhibitors. (Zeger Debyser, Peter Cherepanov, Win Pluymers, and Erik de Clercq). The direction of ribonucleases H by antisense oligodeoxynucleotides. (Richard V. Giles and David M. Tidd). The 2-5A/RNase L Pathway and inhibition by RNase L Inhibitor (RLI). (Catherine Bisbal, Tamim Salehzada, Michelle Silhol, Camille Martinad, Florence Le Roy, and Bernard Lebleu). Crystallization and crystal structure determination of ribomuclease A-Ribonuclease inhibitor protein complex. (Bostjan Kobe). Methods for studying the interaction of barnase with its inhibitor barstar. (Gideon Schreiber). Assaying in vitro refolding of RNases by mass spectrometry. (Gennaro Marino, Piero Pucci, and Margherita Ruoppolo). Dissecting nucleases into their structural and functional domains: mapping the RNA-Binding surface of RNase III by NMR. (Andres Ramos and Annalisa Pastore). Using electrostatics to define the active site of serratia endonuclease. (Kurt L. Krause and Mitchell D. Miller). Homology modeling and simulations of nuclease structures. (Kizhake V. Soman, Catherine H. Schein, Hongyao Zhu, and Werner Braun). Methods for determining activity and specificity of DNA binding and DNA cleavage by class II restriction endonucleases. (Albert Jeltsch and Alfred M. Pingoud). Engineered properties and assays for human DNase I mutants. (Clark Q. Pan, Dominick V. Sinicropi, and Robert A. Lazarus). Assays for human DNase I activity in biological matrices. (Dominick V. Sinicrop and Robert A. Lazarus). Evaluation of retroviral ribonuclease H activity. (Jennifer T. Miller, Jason W. Rausch, and Stuart F. J. Le Grice). Assays for detection of RNase A superfamily robonucleases. (Helene F. Rosenberg and Joseph B. Domachowske). Assay for antitumor and lectin avctivity of angiogenin. (James F. Riordan and Robert Shapiro). Preparation and preclinical characterization of RNase-Based immunofusion proteins. (Dianne L. Newton and Susanna M. Rybak). Restriction endonucleases and their uses. (Raymond J. Williams). Isolation and characterization of an unknown restriction endonuclease. (Raymond J. Williams). Gene modification with hapaxoterministic restriction enzymes: easing the way. (Shelby L. Berger). Techniques to measure nucleic acid-protein binding and specificity: nuclear extract preparations, DNase I footprinting, and mobility shift assays. (Richard A. Rippe, David A. Brenner, and Antonio Tugores). Analysing the development expression of sigma factors with S1-nuclease mapping. (Jan Kormanec). Detection and quantitation of mRNAs using ribonuclease protection assays. (Ellen A. Prediger).