Photosynthesis Research Protocols

Fractionations of Thylakoid membranes into grana and stroma thylakoids; Isolation of photosystem I particles from spinach; Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii; Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants; Isolation of functional photosystem II core particles from the cyanobacterium synechocystis sp. PCC 6803; Isolation of photosystem I reaction center preparation from spinach; Isolation of photosystem II reaction center complexes from plants; Isolation of photosystem I reaxtion center subunit polypeptides form spinach; Purificatoin and crystallizaton of the cytochrome b6f complex in oxygenic photosynthesis; Purification of plastocyanini and cytochorme c6 from plants green algae, and cyanobacteria; Preparation fo native recombinant light-harvesting chlorophyll-a/b complex; Isolation and characterization of lamellar aggregates of LHCII structural transitions; Separation, purification, and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II; Isolation of CP43 and CP47 photosystem II proximal antenna complexes from plants; Determination and quantification of photosynthetic pigments by reserve phase high-performance liquid chromatography, thin-layer chromatography, and spectrophotometry; Isolation and identificaton of chloroplast lipids; DNA adducts with chlorophyll and chlorophyllin as antimutagenic agents: synthesis, stability, and structural features; Incorporation and analysis of LHCII in model systems; Photosystem II reconstitution into proteoliposomes: structure-function characterization; Extraction of the functional manganese and calcium from photosystem II; Assay of photoinhibition of photosystem II and protease activity...