Basics of CRISPR/Cas Mediated Plant Genome Editing

"Basics of CRISPR mediated plant genome editing offers a comprehensive collection of chapters from various authors across the globe. This book contains the basic understanding and development of CRISPR/Cas9/12a/12b for genome editing in plants. Starting from bioinformatics approach to identify guide RNA, off-targets, design gRNA/multiplex gRNA, and an implementation the CRISPR-Cas9 system in different plant species. A clear description of classification of the existing CRISPR system, ortholog of Cas endonucleases, and its application in the development of future genome editing tools. A focus of CRISPR/Cas toolkit and their different delivery methods in different crop and fruit improvement. Another application and case studies of selected medicinal plants will be covered. The step wise information within the book offers an essential guide for graduate and master students, teachers, and scientists in academic and research institute as well as private companies working area of plant science"--