Fluorescence Imaging and Biological Quantification

Cover -- Half Title -- Title Page -- Copyright Page -- Dedication -- Table of Contents -- Preface -- Acknowledgments -- Editors -- Contributors -- Introduction -- SECTION I: ADVANCED METHODS OF ANALYSIS -- 1: Confocal microscopy in the life sciences -- 2: Imaging flow cytometry for quantification of cellular parameters -- 3: Live-cell imaging: Seeing is believing -- 4: Atomic force microscopy: A tool for imaging, manipulating biomolecules, and quantifying single-molecule interactions in biological systems -- 5: Confocal Raman microscopy: Imaging the chemistry -- 6: Tracking cancer: In vivo imaging techniques -- 7: Quantum dots: Concepts, imaging, and therapeutic applications in cancer -- 8: Selecting the appropriate in situ proximity ligation assay protocol -- 9: Cell detection and joint shape tracking using local image filters -- SECTION II: MOLECULAR AND CELLULAR APPLICATIONS -- 10: Studying subcellular signaling events in living microglial cells using fluorescence resonance energy transfer-based nanosensors -- 11: Membrane trafficking under the microscope, what new imaging technologies have brought to light -- 12: Illuminating the cycle of life -- 13: Methods for the visualization of circadian rhythms: From molecules to organisms -- 14: Measurement of lysosomal ion homeostasis by fluorescence microscopy -- 15: Capturing quantitative features of protein expression from in situ fluorescence microscopic images of cancer cell populations -- 16: Cancer cell invadopodia: Visualization and quantification tools -- Index