Hepatocyte transplantation: methods and protocols

Due To Its Efficacy In Animal Models, Cellular Therapy Using Human Hepatocytes Is Being Evaluated Worldwide As An Alternative To Organ Transplantation In Patients With Liver-based Metabolic Disease And Acute Liver Failure. In Hepatocyte Transplantation: Methods And Protocols, An International Panel Of Experts Provide Up-to-date Laboratory And Clinical Techniques Covering The Many Key Areas Necessary For Successful Transplantation, Such As Cryopreservation, Quality Assurance, Detection Of Cell Engraftment, And The Future Of The Field With The Development Of Foetal Hepatoblasts And Stem Cell Derived Hepatocytes. Written In The Highly Successful Methods In Molecular Biology!series Format, The Chapters In This Volume Present Brief Introductions To The Material, Lists Of The Necessary Materials And Reagents, Readily Reproducible, Step-by-step Laboratory Protocols, And Notes Sections Which Highlight Tips On Troubleshooting And Avoiding Known Pitfalls. Comprehensive And Cutting-edge, Hepatocyte Transplantation: Methods And Protocols Is An Ideal Guide For Researchers Setting Out In The Rapidly Progressing Field Of Hepatocyte Transplantation, As Well As Those Who Already Have Experience With This New Therapy For Liver Disease Human Hepatocyte Transplantation Overview / Juliana Puppi And Anil Dhawan -- Isolation Of Human Hepatocytes / Ragai R. Mitry -- An Optimised Method For Cryopreservation Of Human Hepatocytes / Claire Terry And Robin D. Hughes -- Liver Cell Culture Techniques / José V. Castell And María José Gómez-lechón -- In-vitro Assays For Induction Of Drug Metabolism / Brian G. Lake ... [et Al.] -- Hepatocyte Apoptosis / Mustapha Najimi, Françoise Smets, And Etienne Sokal -- Small Animal Models Of Hepatocyte Transplantation / Jurgen Seppen, Ebtisam El Filali, And Ronald Oude Elferink -- Hepatocyte Transplantation Techniques : Large Animal Models / Anne Weber, Marie Thérèse Groyer-picard, And Ibrahim Dagher -- Cell Transplant Techniques : Engraftment Detection Of Cells / Robert A. Fisher And Valeria R. Mas -- Hepatic Preconditioning For Transplanted Cell Engraftment And Proliferation / Yao-ming Wu And Sanjeev Gupta -- Ex Vivo Gene Transfer Into Hepatocytes / Xia Wang ... [et Al.] -- Sources Of Adult Hepatic Stem Cells : Haematopoietic / Rosemary Jeffery, Richard Poulsom, And Malcolm R. Alison -- Production Of Hepatocyte-like Cells From Human Amnion / Toshio Miki ... [et Al.] -- Generation Of Hepatocytes From Human Embryonic Stem Cells / Niloufar Safinia And Stephen L. Minger -- Isolation, In Vitro Cultivation, And Characterisation Of Foetal Liver Cells / Yue Wu, Chetan C. Shatapathy, And Stephen L. Minger -- Human Intrahepatic Biliary Epithelial Cell Lineages : Studies In Vitro / Ruth Joplin And Stivelia Kachilele -- Liver Cell Labelling With Mri Contrast Agents / Michel Modo, Thomas J. Meade, And Ragai R. Mitry -- Microbiological Monitoring Of Hepatocyte Isolation In The Gmp Laboratory / Sharon C. Lehec. Edited By Anil Dhawan, Robin D. Hughes. Includes Bibliographical References And Index.