Human Genome Methods

Human Genome Methods is a practical guide to the application of molecular biology and genetics techniques to research on human cells. Written by recognized authorities who often originated the techniques described, chapters present experimental protocols that are readily used at the laboratory bench. The step-by-step protocols are concise and easy to follow to be reproducible by researchers of various levels of expertise. Suggestions for successful application of procedures are included, along with recommended materials and suppliers. Helpful background information and results of applying the methods described are also given. Section I covers topics such as microsatellite DNA, dynamic mutations, gene targeting using the DNA triple helix, and protease footprinting of DNA-protein interactions. This is followed in Section II by discussions of in situ hybridization, cell synchronization, and cell cycle specific gene expression. Methods concerned with programmed cell death are explored in Section III, which covers this emerging research area and the culture and analysis of cancer cells. Section IV presents methods related to transgene analysis of mouse embryonic stem cells, generation and knockout studies with null mutant mice, and mouse models for human disease. The final section reviews genome mapping, with an emphasis on the construction of linkage maps and on somatic cell hybrids for mapping disease genes.