In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development

Cover -- Half Title -- Title Page -- Copyright Page -- Contents -- Preface -- Chapter 1: Introduction to Anticancer Drug Development -- 1.1 Cancer -- 1.2 Chemotherapy -- 1.3 Need for Effective Anticancer DrugS -- 1.4 Stages of Anticancer Drug Development -- References -- Chapter 2: In Vitro Biological Assays for Anticancer Chemotherapeutics -- 2.1 Introduction -- 2.2 Advantages of in Vitro Assays -- 2.3 Disadvantages of in Vitro Assays -- 2.4 Classification of in Vitro Assays -- 2.5 Essential Techniques of Cell Cultures -- 2.5.1 Primary Cell Cultures -- 2.5.2 Maintenance of Cell Lines -- 2.5.3 Subculture of Cells -- References -- Chapter 3: Cell Viability and Cytotoxicity Assays -- 3.1 Introduction -- 3.2 Cell Membrane Integrity Assays -- 3.2.1 Dye Exclusion Assays -- 3.2.1.1 Trypan Blue Assay -- 3.2.1.2 Calcein Acetoxymethyl (Calcein-AM) Assay -- 3.2.1.3 Propidium Iodide (PI)/7-Aminoactinomycin D (7-AAD) Staining Assay -- 3.2.2 LDH Release Assays -- 3.3 Functional or Metabolic Assays -- 3.3.1 Tetrazolium Reduction Assays -- 3.3.1.1 MTT Assay -- 3.3.1.2 MTS Assay -- 3.3.1.3 XTT Assay -- 3.3.1.4 WST Assay -- 3.3.2 Sulforhodamine B Assay -- 3.3.3 Acid Phosphatase Assay -- 3.3.4 Alamar Blue Oxidation-Reduction Assay -- 3.3.5 Neutral Red Assay -- 3.3.6 Protease Viability Marker Assay -- 3.4 Cell Adhesion Assay -- References -- Chapter 4: Cell Proliferation Assays -- 4.1 Introduction -- 4.2 DNA Labeling Assays -- 4.2.1 [3.H]-Thymidine Assay -- 4.2.2 BrdU Incorporation Assay -- 4.3 Metabolic Assays -- 4.3.1 Dye Assays -- 4.3.2 ATP Assays -- 4.4 Clonogenic Reproductive Assay -- References -- Chapter 5: Apoptosis Assays -- 5.1 Introduction -- 5.2 Light Microscopy -- 5.3 Western Blot Caspase Activation Assay -- 5.4 Annexin-V Staining -- 5.5 DNA Ladder Assay -- 5.6 ELISA -- 5.7 TUNEL Assay -- 5.8 Comet Assay