Microbial Biofilms Omics Biology, Antimicrobials and Clinical Implications

Biofilm Proteomics Studies on Extracellular Polymeric Substances -- Biofilm Proteomics Studies of Oxidative Stress Response (Increased Anti-Oxidative Activity) -- Fungal biofilm proteomics -- Drug-response proteomics of biofilms -- Study of post-translational modifications in biofilms -- Metaproteomics studies of biofilms -- Concluding remarks -- Corresponding Author -- References -- Chapter 7: Metabolomics of Microbial Biofilms -- Introduction -- Metabolomics Approaches for Biofilm Characterisation -- Overall Workflow of Metabolomics -- Sample Preparation for Metabolomics Analysis -- Modes of Metabolomics Analysis -- Instrumentation for Metabolomics Study -- MS Instrumentation -- LC-MS -- LC-MS Ionisation Sources -- ESI -- APCI -- APPI -- GC-MS and GC/GC-MS -- MS Mass Analysers -- Quadrupole Analysers -- TOF Analysers -- Ion Trap Analysers -- Hybrid Analysers -- LC-MS versus GC-MS -- NMR -- MS versus NMR -- Data Analysis and Interpretation -- Biofilm Metabolomics: Practical Applications -- Extracellular Polymeric Substances in Biofilms -- Challenges in EPS Extraction and Analysis -- EPS Extraction from Biofilms -- Profiling of Planktonic versus Biofilm Cells -- Biofilm Metabolomics: Case Studies -- Metabolome Analysis of Oral Biofilms Using a Combination of Capillary Electrophoresis and TOF MS -- Metabolic Differentiation of Planktonic and Biofilm Modes of S. aureus -- Future Directions -- Corresponding author -- References -- Chapter 8: Biofilm Persisters -- Introduction -- Type I and Type II Persisters -- Environmental Triggers of Persister Cell Formation -- Mechanisms behind Microbial Persistence -- Persisters in Biofilms -- Combating Persisters and Biofilm-Related Infections -- Conclusions -- Corresponding author -- References -- Chapter 9: Host-Biofilm Interactions at Mucosal Surfaces and Implications in Human Health -- Introduction