Nonradioactive Labeling and Detection of Biomolecules

This study gathers together all of the important nonradioactive labelling techniques for nucleic acids, proteins, glycoproteins and glycolipids like DIG, Biotin, BrdU, Sulfone, Immunogold, Silver Enhancement and SNAP, as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labelled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, and also non-radioactive techniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with lab protocols, trouble-shooting tips and references.