Quantitative Bioassay

Details the how's and why's of three principal types of assay procedure: those using animals or their tissues, assays using microorganisms, and immunoassays. More than half the book is devoted to the latter, since classical assays are being replaced by an ever-growing range of immunoassay techniques. Covers basic principles of bioassays, including biological activity, standard materials, and experimental design, as well as basic approaches to microbiological assays, including plate assays and tube assays. The in-depth treatment of immunological assays includes discussion of polyclonal antibodies, monoclonal antibodies, binding procedures, labelling, separation procedures, and evaluation.