RNA Interference (RNAi) Nuts & Bolts of RNAi Technology

This work focuses on practical approaches to and uses of a revolutionary technology called RNAi for RNA interference. A recently developed research tool, this process allows scientists to knock out or knock down the levels of a gene product, shut off targeted genes in an organism, and then monitor the results within that organism. Leading academic and biotechnology researchers in various fields that utilize this process introduce and explain a specific model system and use of RNA interference as well as necessary background information. Tools for the Arabidopsis, Caenrhabditis, and Drosophila model organism systems are offered, as is an overview of siRNA design and RNA transfection processes in mammalian cells. A discussion of chemical and enzymatic synthesis and preparation of siRNA in vitro is also included.