Standard Operational Procedures in Reproductive Medicine Laboratory and Clinical Practice

Cover -- Half Title -- Title Page -- Copyright Page -- Contents -- About the Editors -- List of Contributors -- Section I: Laboratory Procedures -- 1. The Documentation System -- 2. Culture Media Techniques -- 3. IVF Incubator Handling -- 4. Preparation for Follicle Aspiration and Isolation of Cumulus-Oocyte-Complexes (COC) -- 5. Semen Collection and Sample Reception -- 6. Spermiogramm, Makler Chamber, Neubauer Chamber, CASA -- 7. Sperm Viability Testing -- 8. Sperm Preparation-Swim Up, Density Gradient, Migration Chamber, Zeta Method -- 9. Sperm Selection Using Hyaluronan Binding -- 10. IVF-Insemination -- 11. ICSI -- 12. Oocyte In Vitro Maturation-Isolation of COCs and Culture for Maturation -- 13. Artificial Oocyte Activation -- 14. Cryopreservation-Sperm TESE and MESA -- 15. Vitrification-Oocytes, 2PN, Embryos, Blastocysts -- 16. Slow Freezing: Principles, Oocytes, 2PN, Embryos, Blastocysts -- 17. Oocyte Selection -- 18. Zygote Selection -- 19. Selection and Grading of Embryos on Day 2/3 and Blastocysts on Day 5/6 -- 20. Time-Lapse Imaging -- 21. Assisted Hatching -- 22. Zona Manipulation at Various Embryo Stages: Mechanical/Acidic Medium -- 23. Biopsy: Polar Body, Blastomere, Trophectoderm -- 24. Sampling of Cells after Biopsy for aCGH -- 25. Embryo Transfer-Standard and Ultrasound Guided -- 26. Hygienic Standards in the IVF Laboratory -- 27. Quality Control of IVF Laboratory Supplies-Mouse Embryo Assay (MEA) -- 28. Troubleshooting in the IVF Laboratory -- 29. Microscopy -- 30. Fertility Preservation -- Section II: Clinical Procedures -- 31. Ultrasound Evaluation in Treatment Management -- 32. Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies -- 33. Infertility Ultrasound Evaluation: One-Stop Procedure -- 34. Office Hysteroscopy -- 35. Hysteroscopic Management of Uterine Septum