A guide to handling giant polytene and lampbrush chromosomes, preparing chromatin, visualizing transcription, locating genes on chromosomes, identifing fine-scale polymorphisms on mitotic chromosomes, etc. Covers many specialized chromosome techniques; each method is tested and reproducible and comes with a model protocol. This 2nd Edition has several new sections on recently developed cytological techniques including spreading of synaptonemal complexes from cells in meiotic prophase, banding of mitotic chromosomes with Giemsa and fluorochromes, indirect immunofluorescence, lampbrush chromosomes from Xenopus laevis, and isolation of polytene chromosomes. Also covers new legislation affecting those who work with animal chromosomes.
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